Coding

Part:BBa_K1199043:Design

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-16)


DhaA31 TCP(trichlorophenol)->2,3-DCP(2,3-dichloropropano)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 744
    Illegal NgoMIV site found at 870
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no restriction site contained


Source

DhaA is from Rhodococcus sp hydrolyzes

References

1 Maryna Lahoda, Radka Chaloupkova, Alena Stsiapanava, Jiri Damborsky, and Ivana Kuta Smatanovaa, Crystallization and crystallographic analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA mutant DhaA31 and its complex with 1,2,3-trichloropropane ,Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011 February 25 .
2 Fabritz S, Maaß F, Avrutina O, Heiseler T, Steinmann B, Kolmar H, A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay. AMB Express. 2012 Sep 24.